We have formulated a unique serum-free base media (SFBM), which together with our supplement (human fibroblast conditioned media, hFCM), supports the growth and expansion of human ASCs in vitro. Adipose stem/stromal cells (ASCs) are a type of adult mesenchymal stem cells (MSCs) that can be easily isolated from adipose tissue. ASCs can be cultured in vitro over several passages, but conventional culture conditions involve the use of human or animal serum to enhance cell attachment and promote cell survival and proliferation. Since these cells or their conditioned media, which contains many beneficial secretory factors, are increasingly being used in regenerative medicine, it is crucial to eliminate serum supplements to ensure scalability and prevent adverse immune reactions.Our complete serum-free media (SFM) supports the growth and proliferation of ASCs up to passage 14 in vitro while maintaining their mesenchymal stem cell characteristics and stemness. These cells can be successfully cultured in monolayers and three-dimensional scaffolds, and are able to differentiate into adipocytes, chondrocytes and osteocytes. In addition, they secrete less pro-inflammatory chemokines, unlike ASCs cultured in conventional media supplemented with 2% human serum. These ASCs or their conditioned media, which exhibits biological activity, may thus be safely used for downstream cosmetic or regenerative applications without the limitations, risks, or adverse reactions that may be associated with human serum. As with all our products safety and efficacy are our top priorities.
Here are some pictures of undifferentiated ASCs:
ASCs maintain their differentiation potential